Cargando…
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
In vivo mutagenesis systems accelerate directed protein evolution but often show restricted capabilities and deleterious off-site mutations on cells. To overcome these limitations, here we report an in vivo platform to diversify specific DNA segments based on protein fusions between various base dea...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755918/ https://www.ncbi.nlm.nih.gov/pubmed/33353963 http://dx.doi.org/10.1038/s41467-020-20230-z |