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Engineering of Thermostable β‐Hydroxyacid Dehydrogenase for the Asymmetric Reduction of Imines

The β‐hydroxyacid dehydrogenase from Thermocrinus albus (Ta‐βHAD), which catalyzes the NADP(+)‐dependent oxidation of β‐hydroxyacids, was engineered to accept imines as substrates. The catalytic activity of the proton‐donor variant K189D was further increased by the introduction of two nonpolar flan...

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Detalles Bibliográficos
Autores principales: Stockinger, Peter, Schelle, Luca, Schober, Benedikt, Buchholz, Patrick C. F., Pleiss, Jürgen, Nestl, Bettina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756219/
https://www.ncbi.nlm.nih.gov/pubmed/32939899
http://dx.doi.org/10.1002/cbic.202000526