Cargando…
Engineering of Thermostable β‐Hydroxyacid Dehydrogenase for the Asymmetric Reduction of Imines
The β‐hydroxyacid dehydrogenase from Thermocrinus albus (Ta‐βHAD), which catalyzes the NADP(+)‐dependent oxidation of β‐hydroxyacids, was engineered to accept imines as substrates. The catalytic activity of the proton‐donor variant K189D was further increased by the introduction of two nonpolar flan...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756219/ https://www.ncbi.nlm.nih.gov/pubmed/32939899 http://dx.doi.org/10.1002/cbic.202000526 |