Quantification of DNA methylation independent of sodium bisulfite conversion using methylation‐sensitive restriction enzymes and digital PCR

Epigenetic regulation is important in human health and disease, but the exact mechanisms remain largely enigmatic. DNA methylation represents one epigenetic aspect but is challenging to quantify. In this study, we introduce a digital approach for the quantification of the amount and density of DNA m...

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Detalles Bibliográficos
Autores principales: Nell, Rogier J., van Steenderen, Debby, Menger, Nino V., Weitering, Thomas J., Versluis, Mieke, van der Velden, Pieter A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756443/
https://www.ncbi.nlm.nih.gov/pubmed/32906203
http://dx.doi.org/10.1002/humu.24111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756443/
https://www.ncbi.nlm.nih.gov/pubmed/32906203
http://dx.doi.org/10.1002/humu.24111

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