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Functional Multiple-Spine Calcium Imaging from Brain Slices

Most excitatory inputs arrive at dendritic spines in a postsynaptic neuron. To understand dendritic information processing, it is critical to scrutinize the spatiotemporal dynamics of synaptic inputs along dendrites. This protocol combines spinning-disk confocal imaging with whole-cell patch-clamp r...

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Detalles Bibliográficos
Autores principales: Ishikawa, Tomoe, Kobayashi, Chiaki, Takahashi, Naoya, Ikegaya, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756976/
https://www.ncbi.nlm.nih.gov/pubmed/33377015
http://dx.doi.org/10.1016/j.xpro.2020.100121