Single-Cell Sorting of HBsAg-Binding Memory B Cells from Human Peripheral Blood Mononuclear Cells and Antibody Cloning

The isolation of human antibodies with naturally paired heavy and light chains is crucial for understanding the human antibody immune response. Here, we present a protocol for antibody cloning from the sorted single human memory B cells recognizing hepatitis B virus (HBV) S antigen (HBsAg). A two-fl...

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Detalles Bibliográficos
Autores principales: Zhou, Yunjiao, Liu, Zhenmi, Wang, Zijun, Zhang, Qianqian, Mayer, Christian T., Schoofs, Till, Nussenzweig, Michel C., de Jong, Ype P., Wang, Qiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757113/
https://www.ncbi.nlm.nih.gov/pubmed/33377023
http://dx.doi.org/10.1016/j.xpro.2020.100129

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757113/
https://www.ncbi.nlm.nih.gov/pubmed/33377023
http://dx.doi.org/10.1016/j.xpro.2020.100129

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