Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17(+) hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The gener...

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Detalles Bibliográficos
Autores principales: Nafria, Monica, Bonifer, Constanze, Stanley, Edouard Guy, Ng, Elizabeth Siewsun, Elefanty, Andrew George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757115/
https://www.ncbi.nlm.nih.gov/pubmed/33377024
http://dx.doi.org/10.1016/j.xpro.2020.100130
Descripción
Sumario:This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17(+) hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45(+)CD34(+)RUNX1C(+) progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).

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