Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17(+) hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The gener...

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Detalles Bibliográficos
Autores principales: Nafria, Monica, Bonifer, Constanze, Stanley, Edouard Guy, Ng, Elizabeth Siewsun, Elefanty, Andrew George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757115/
https://www.ncbi.nlm.nih.gov/pubmed/33377024
http://dx.doi.org/10.1016/j.xpro.2020.100130
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author Nafria, Monica
Bonifer, Constanze
Stanley, Edouard Guy
Ng, Elizabeth Siewsun
Elefanty, Andrew George
author_facet Nafria, Monica
Bonifer, Constanze
Stanley, Edouard Guy
Ng, Elizabeth Siewsun
Elefanty, Andrew George
author_sort Nafria, Monica
collection PubMed
description This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17(+) hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45(+)CD34(+)RUNX1C(+) progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).
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spelling pubmed-77571152020-12-28 Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells Nafria, Monica Bonifer, Constanze Stanley, Edouard Guy Ng, Elizabeth Siewsun Elefanty, Andrew George STAR Protoc Protocol This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17(+) hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45(+)CD34(+)RUNX1C(+) progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020). Elsevier 2020-10-16 /pmc/articles/PMC7757115/ /pubmed/33377024 http://dx.doi.org/10.1016/j.xpro.2020.100130 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nafria, Monica
Bonifer, Constanze
Stanley, Edouard Guy
Ng, Elizabeth Siewsun
Elefanty, Andrew George
Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title_full Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title_fullStr Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title_full_unstemmed Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title_short Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
title_sort protocol for the generation of definitive hematopoietic progenitors from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757115/
https://www.ncbi.nlm.nih.gov/pubmed/33377024
http://dx.doi.org/10.1016/j.xpro.2020.100130
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