Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers

We describe an RT-PCR protocol that allows high-resolution mapping of splicing products and isoforms using fluorescently labeled primers. Each species contains one fluorescent group allowing a direct comparison of the different isoforms despite size differences. A custom-size ladder enables the prec...

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Detalles Bibliográficos
Autores principales: Weathers, Indya, Gabunilas, Jason, Samson, Joyce, Roy, Kevin, Chanfreau, Guillaume F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757285/
https://www.ncbi.nlm.nih.gov/pubmed/33377034
http://dx.doi.org/10.1016/j.xpro.2020.100140

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757285/
https://www.ncbi.nlm.nih.gov/pubmed/33377034
http://dx.doi.org/10.1016/j.xpro.2020.100140

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