Cargando…
Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers
We describe an RT-PCR protocol that allows high-resolution mapping of splicing products and isoforms using fluorescently labeled primers. Each species contains one fluorescent group allowing a direct comparison of the different isoforms despite size differences. A custom-size ladder enables the prec...
Autores principales: | Weathers, Indya, Gabunilas, Jason, Samson, Joyce, Roy, Kevin, Chanfreau, Guillaume F. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757285/ https://www.ncbi.nlm.nih.gov/pubmed/33377034 http://dx.doi.org/10.1016/j.xpro.2020.100140 |
Ejemplares similares
-
Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae
por: Gabunilas, Jason, et al.
Publicado: (2016) -
Widespread Use of Non-productive Alternative Splice Sites in Saccharomyces cerevisiae
por: Kawashima, Tadashi, et al.
Publicado: (2014) -
PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
por: Tokheim, Collin, et al.
Publicado: (2014) -
Common genomic elements promote transcriptional and DNA replication roadblocks
por: Roy, Kevin, et al.
Publicado: (2016) -
Mutations of EXOSC3/Rrp40p associated with neurological diseases impact ribosomal RNA processing functions of the exosome in S. cerevisiae
por: Gillespie, Abby, et al.
Publicado: (2017)