Protocol for Proximity-Dependent Proteomic Profiling in Yeast Cells by APEX and Alk-Ph Probe

Alk-Ph is a clickable APEX2 substrate developed for spatially restricted protein/RNA labeling in intact yeast cells. Alk-Ph is more water soluble and cell wall permeable than biotin-phenol substrate, allowing more efficient profiling of the subcellular proteome in microorganisms. We describe the pro...

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Detalles Bibliográficos
Autores principales: Li, Yi, Liu, Keke, Zhou, Ying, Yang, Jing, Zou, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757286/
https://www.ncbi.nlm.nih.gov/pubmed/33377031
http://dx.doi.org/10.1016/j.xpro.2020.100137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757286/
https://www.ncbi.nlm.nih.gov/pubmed/33377031
http://dx.doi.org/10.1016/j.xpro.2020.100137

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