Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells

Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenche...

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Detalles Bibliográficos
Autores principales: Kitchen, Philip, Salman, Mootaz M., Abir-Awan, Mohammed, Al-Jubair, Tamim, Törnroth- Horsefield, Susanna, Conner, Alex C., Bill, Roslyn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757292/
https://www.ncbi.nlm.nih.gov/pubmed/33377051
http://dx.doi.org/10.1016/j.xpro.2020.100157

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757292/
https://www.ncbi.nlm.nih.gov/pubmed/33377051
http://dx.doi.org/10.1016/j.xpro.2020.100157

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