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Determining Alternative Protein Isoform Expression Using RNA Sequencing and Mass Spectrometry

Alternative splicing greatly expands the coding capacity of the human genome, but how many alternative transcripts are translated as proteins or carry functional importance remains unknown and awaits experimental verification. Here, we describe a protocol that combines transcriptomics (RNA-seq) and...

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Detalles Bibliográficos
Autores principales: Han, Yu, Wright, Julianna M., Lau, Edward, Lam, Maggie Pui Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757315/
https://www.ncbi.nlm.nih.gov/pubmed/33377032
http://dx.doi.org/10.1016/j.xpro.2020.100138