Transduction of Primary AML Cells with Lentiviral Vector for In Vitro Study or In Vivo Engraftment

We describe a method to silence genes in primary acute myeloid leukemia cells by transducing them with shRNA in lentiviral vectors. The transduction of primary non-adherent cells is particularly challenging. The protocol will aid in performing such experiments and is particularly helpful to prepare...

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Detalles Bibliográficos
Autores principales: Schimmer, Aaron D., Singh, Rashim Pal, Seneviratne, Ayesh K., Thomas, Geethu E., MacLean, Neil, Hurren, Rose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757357/
https://www.ncbi.nlm.nih.gov/pubmed/33377057
http://dx.doi.org/10.1016/j.xpro.2020.100163

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757357/
https://www.ncbi.nlm.nih.gov/pubmed/33377057
http://dx.doi.org/10.1016/j.xpro.2020.100163

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