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Transduction of Primary AML Cells with Lentiviral Vector for In Vitro Study or In Vivo Engraftment
We describe a method to silence genes in primary acute myeloid leukemia cells by transducing them with shRNA in lentiviral vectors. The transduction of primary non-adherent cells is particularly challenging. The protocol will aid in performing such experiments and is particularly helpful to prepare...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757357/ https://www.ncbi.nlm.nih.gov/pubmed/33377057 http://dx.doi.org/10.1016/j.xpro.2020.100163 |