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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined si...

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Detalles Bibliográficos
Autores principales: Petropavlovskiy, Andrey A., Tauro, Michael G., Lajoie, Patrick, Duennwald, Martin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757406/
https://www.ncbi.nlm.nih.gov/pubmed/33377076
http://dx.doi.org/10.1016/j.xpro.2020.100182