A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined si...

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Detalles Bibliográficos
Autores principales: Petropavlovskiy, Andrey A., Tauro, Michael G., Lajoie, Patrick, Duennwald, Martin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757406/
https://www.ncbi.nlm.nih.gov/pubmed/33377076
http://dx.doi.org/10.1016/j.xpro.2020.100182
Descripción
Sumario:We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).

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