A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined si...

Descripción completa

Detalles Bibliográficos
Autores principales: Petropavlovskiy, Andrey A., Tauro, Michael G., Lajoie, Patrick, Duennwald, Martin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757406/
https://www.ncbi.nlm.nih.gov/pubmed/33377076
http://dx.doi.org/10.1016/j.xpro.2020.100182
_version_ 1783626745329483776
author Petropavlovskiy, Andrey A.
Tauro, Michael G.
Lajoie, Patrick
Duennwald, Martin L.
author_facet Petropavlovskiy, Andrey A.
Tauro, Michael G.
Lajoie, Patrick
Duennwald, Martin L.
author_sort Petropavlovskiy, Andrey A.
collection PubMed
description We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).
format Online
Article
Text
id pubmed-7757406
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-77574062020-12-28 A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates Petropavlovskiy, Andrey A. Tauro, Michael G. Lajoie, Patrick Duennwald, Martin L. STAR Protoc Protocol We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020). Elsevier 2020-11-25 /pmc/articles/PMC7757406/ /pubmed/33377076 http://dx.doi.org/10.1016/j.xpro.2020.100182 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Petropavlovskiy, Andrey A.
Tauro, Michael G.
Lajoie, Patrick
Duennwald, Martin L.
A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title_full A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title_fullStr A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title_full_unstemmed A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title_short A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
title_sort quantitative imaging-based protocol for yeast growth and survival on agar plates
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757406/
https://www.ncbi.nlm.nih.gov/pubmed/33377076
http://dx.doi.org/10.1016/j.xpro.2020.100182
work_keys_str_mv AT petropavlovskiyandreya aquantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT tauromichaelg aquantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT lajoiepatrick aquantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT duennwaldmartinl aquantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT petropavlovskiyandreya quantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT tauromichaelg quantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT lajoiepatrick quantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates
AT duennwaldmartinl quantitativeimagingbasedprotocolforyeastgrowthandsurvivalonagarplates

Ejemplares similares