De Novo Generation of Murine and Human MADR Recipient Cell Lines for Locus-Specific, Stable Integration of Transgenic Elements

Mosaic analysis by dual recombinase-mediated cassette exchange (MADR) is a technology that allows stable and locus-specific integration of transgenic elements into recipient cells carrying loxP and FRT sites. Nevertheless, most cell lines lack these recombination-specific sites. This protocol descri...

Descripción completa

Detalles Bibliográficos
Autores principales: Ayala-Sarmiento, Alberto E., Kobritz, Naomi, Breunig, Joshua J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757409/
https://www.ncbi.nlm.nih.gov/pubmed/33377078
http://dx.doi.org/10.1016/j.xpro.2020.100184

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757409/
https://www.ncbi.nlm.nih.gov/pubmed/33377078
http://dx.doi.org/10.1016/j.xpro.2020.100184

Ejemplares similares