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De Novo Generation of Murine and Human MADR Recipient Cell Lines for Locus-Specific, Stable Integration of Transgenic Elements
Mosaic analysis by dual recombinase-mediated cassette exchange (MADR) is a technology that allows stable and locus-specific integration of transgenic elements into recipient cells carrying loxP and FRT sites. Nevertheless, most cell lines lack these recombination-specific sites. This protocol descri...
Autores principales: | Ayala-Sarmiento, Alberto E., Kobritz, Naomi, Breunig, Joshua J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757409/ https://www.ncbi.nlm.nih.gov/pubmed/33377078 http://dx.doi.org/10.1016/j.xpro.2020.100184 |
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