Generation of Casp8(FL122/123GG) Mice Using CRISPR-Cas9 Technology

The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8(FL122/123GG) missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details...

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Detalles Bibliográficos
Autores principales: Pelletier, Stephane, Tummers, Bart, Green, Douglas R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757410/
https://www.ncbi.nlm.nih.gov/pubmed/33377075
http://dx.doi.org/10.1016/j.xpro.2020.100181
Descripción
Sumario:The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8(FL122/123GG) missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details on the use and execution of this protocol, please refer to Tummers et al. (2020).

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