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Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus

Translation of distally localized mRNAs is an evolutionary mechanism occurring in polarized cells. It has been observed in astrocytes, whose processes contact blood vessels and synapses. Here, we describe a protocol for the purification of the entire pool of ribosome-bound mRNAs in perisynaptic astr...

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Detalles Bibliográficos
Autores principales: Mazaré, Noémie, Oudart, Marc, Cheung, Giselle, Boulay, Anne-Cécile, Cohen-Salmon, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757553/
https://www.ncbi.nlm.nih.gov/pubmed/33377092
http://dx.doi.org/10.1016/j.xpro.2020.100198