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A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons
Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microscopic protr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757556/ https://www.ncbi.nlm.nih.gov/pubmed/33377083 http://dx.doi.org/10.1016/j.xpro.2020.100189 |
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author | Di Nardo, Alessia Vasquez, Sara Sahin, Mustafa |
author_facet | Di Nardo, Alessia Vasquez, Sara Sahin, Mustafa |
author_sort | Di Nardo, Alessia |
collection | PubMed |
description | Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microscopic protrusions are crucial given the role of cilia in the brain. We developed a cell-based assay to monitor cilia in rat hippocampal neurons using lentiviral-mediated shRNA-based gene silencing. This optimized platform can be used for high-throughput cilia imaging, disease modeling, and drug screening. For complete details on the use and execution of this protocol, please refer to Di Nardo et al. (2020). |
format | Online Article Text |
id | pubmed-7757556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77575562020-12-28 A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons Di Nardo, Alessia Vasquez, Sara Sahin, Mustafa STAR Protoc Protocol Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders. Efforts for automated imaging of these microscopic protrusions are crucial given the role of cilia in the brain. We developed a cell-based assay to monitor cilia in rat hippocampal neurons using lentiviral-mediated shRNA-based gene silencing. This optimized platform can be used for high-throughput cilia imaging, disease modeling, and drug screening. For complete details on the use and execution of this protocol, please refer to Di Nardo et al. (2020). Elsevier 2020-12-03 /pmc/articles/PMC7757556/ /pubmed/33377083 http://dx.doi.org/10.1016/j.xpro.2020.100189 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Di Nardo, Alessia Vasquez, Sara Sahin, Mustafa A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title_full | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title_fullStr | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title_full_unstemmed | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title_short | A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons |
title_sort | cell-based assay optimized for high-content cilia imaging with primary rat hippocampal neurons |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757556/ https://www.ncbi.nlm.nih.gov/pubmed/33377083 http://dx.doi.org/10.1016/j.xpro.2020.100189 |
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