Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3(+) Treg Cells by Multiparameter Flow Cytometry

Assessing IL-2-induced phosopho-STAT5 (pSTAT5) content can reveal the cytokine responsiveness of individual T cells. Identifying distinct T cell subsets by nuclear transcription factors, such as Foxp3, and concurrently quantifying intracellular pSTAT5, however, has been technically challenging. Conv...

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Detalles Bibliográficos
Autores principales: Li, Can, Park, Jung-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757559/
https://www.ncbi.nlm.nih.gov/pubmed/33377089
http://dx.doi.org/10.1016/j.xpro.2020.100195

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757559/
https://www.ncbi.nlm.nih.gov/pubmed/33377089
http://dx.doi.org/10.1016/j.xpro.2020.100195

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