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Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3(+) Treg Cells by Multiparameter Flow Cytometry
Assessing IL-2-induced phosopho-STAT5 (pSTAT5) content can reveal the cytokine responsiveness of individual T cells. Identifying distinct T cell subsets by nuclear transcription factors, such as Foxp3, and concurrently quantifying intracellular pSTAT5, however, has been technically challenging. Conv...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757559/ https://www.ncbi.nlm.nih.gov/pubmed/33377089 http://dx.doi.org/10.1016/j.xpro.2020.100195 |