Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR)

This protocol focuses on the cloning and stable integration of sequences of interest by the use of a mosaic analysis with dual recombinases (MADR) plasmid that includes fusion proteins or independent proteins under the control of 2A peptide or IRES elements. Additionally, we describe how to generate...

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Detalles Bibliográficos
Autores principales: Rincon Fernandez Pacheco, David, Sabet, Sara, Breunig, Joshua J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757563/
https://www.ncbi.nlm.nih.gov/pubmed/33377093
http://dx.doi.org/10.1016/j.xpro.2020.100199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757563/
https://www.ncbi.nlm.nih.gov/pubmed/33377093
http://dx.doi.org/10.1016/j.xpro.2020.100199

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