Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using Cryo-Ultramicrotomy

We recently reported that Viburnum tinus fruit generates its metallic blue color using globular lipid inclusions embedded in its epicarpal cell walls. This protocol describes steps to visualize the lipidic nature of the nanostructure using cryo-ultramicrotomy, chloroform extraction, and transmission...

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Detalles Bibliográficos
Autores principales: Sinnott-Armstrong, Miranda, Vignolini, Silvia, Ogawa, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757564/
https://www.ncbi.nlm.nih.gov/pubmed/33377095
http://dx.doi.org/10.1016/j.xpro.2020.100201

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757564/
https://www.ncbi.nlm.nih.gov/pubmed/33377095
http://dx.doi.org/10.1016/j.xpro.2020.100201

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