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Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using Cryo-Ultramicrotomy
We recently reported that Viburnum tinus fruit generates its metallic blue color using globular lipid inclusions embedded in its epicarpal cell walls. This protocol describes steps to visualize the lipidic nature of the nanostructure using cryo-ultramicrotomy, chloroform extraction, and transmission...
Autores principales: | Sinnott-Armstrong, Miranda, Vignolini, Silvia, Ogawa, Yu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757564/ https://www.ncbi.nlm.nih.gov/pubmed/33377095 http://dx.doi.org/10.1016/j.xpro.2020.100201 |
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