Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease

We present a protocol for isolating hepatocytes from mice with established non-alcoholic fatty liver disease. This protocol consists of liver perfusion using a peristaltic pump, followed by a modified 25% and 90% Percoll gradient centrifugation protocol to capture lipid-laden hepatocytes that are us...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Yunshin, Zhao, Meng, Svensson, Katrin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757664/
https://www.ncbi.nlm.nih.gov/pubmed/33377114
http://dx.doi.org/10.1016/j.xpro.2020.100222

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757664/
https://www.ncbi.nlm.nih.gov/pubmed/33377114
http://dx.doi.org/10.1016/j.xpro.2020.100222

Ejemplares similares