Cargando…

Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease

We present a protocol for isolating hepatocytes from mice with established non-alcoholic fatty liver disease. This protocol consists of liver perfusion using a peristaltic pump, followed by a modified 25% and 90% Percoll gradient centrifugation protocol to capture lipid-laden hepatocytes that are us...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jung, Yunshin, Zhao, Meng, Svensson, Katrin J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757664/ https://www.ncbi.nlm.nih.gov/pubmed/33377114 http://dx.doi.org/10.1016/j.xpro.2020.100222

Ejemplares similares

Mapping transcriptional heterogeneity and metabolic networks in fatty livers at single-cell resolution
por: Coassolo, Laetitia, et al.
Publicado: (2022)

Regulation of Energy Metabolism by Receptor Tyrosine Kinase Ligands
por: Zhao, Meng, et al.
Publicado: (2020)

Protocol for Primary Mouse Hepatocyte Isolation
por: Charni-Natan, Meital, et al.
Publicado: (2020)

Toyocamycin attenuates free fatty acid-induced hepatic steatosis and apoptosis in cultured hepatocytes and ameliorates nonalcoholic fatty liver disease in mice
por: Takahara, Ikuko, et al.
Publicado: (2017)

Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes
por: Oeckl, Josef, et al.
Publicado: (2020)

Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease
por: Cansby, Emmelie, et al.
Publicado: (2018)

Correction: Toyocamycin attenuates free fatty acid-induced hepatic steatosis and apoptosis in cultured hepatocytes and ameliorates nonalcoholic fatty liver disease in mice
por: Takahara, Ikuko, et al.
Publicado: (2020)

Establishment of Orthotopic Liver Tumors by Surgical Intrahepatic Tumor Injection in Mice with Underlying Non-Alcoholic Fatty Liver Disease
por: Brown, Zachary J., et al.
Publicado: (2018)

Micropatterned primary hepatocyte co-culture (HEPATOPAC) for fatty liver disease modeling and drug screening
por: Cottier, Karissa E., et al.
Publicado: (2023)

In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration
por: Hu, Chenxia, et al.
Publicado: (2015)

Non-alcoholic fatty liver disease in mice with hepatocyte-specific deletion of mitochondrial fission factor
por: Takeichi, Yukina, et al.
Publicado: (2021)

Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
por: Toda, Gotaro, et al.
Publicado: (2020)

Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues
por: Zhao, Meng, et al.
Publicado: (2023)

Phenotypic Alteration of Hepatocytes in Non-Alcoholic Fatty Liver Disease
por: Lakhani, Hari Vishal, et al.
Publicado: (2018)

Hepatocyte mARC1 promotes fatty liver disease
por: Lewis, Lara C., et al.
Publicado: (2023)

Itaconic acid underpins hepatocyte lipid metabolism in non-alcoholic fatty liver disease in male mice
por: Weiss, Jonathan M., et al.
Publicado: (2023)

Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
por: Li, Yumei, et al.
Publicado: (2022)

Quantifying mitochondrial redox and bilirubin content in intact primary hepatocytes of obese mice using fluorescent reporters
por: Belmas, Thomas, et al.
Publicado: (2023)

The protective roles of augmenter of liver regeneration in hepatocytes in the non-alcoholic fatty liver disease
por: Dong, Yuan, et al.
Publicado: (2022)

Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection
por: Wang, Shuang, et al.
Publicado: (2021)

Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
por: Villanueva-Carmona, Teresa, et al.
Publicado: (2023)

Protocol for measuring intrahepatic triglyceride content in adults with non-alcohol fatty liver disease
por: Ezpeleta, Mark, et al.
Publicado: (2023)

Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
por: Güler, Baran E., et al.
Publicado: (2021)

Hepatocyte caveolin-1 modulates metabolic gene profiles and functions in non-alcoholic fatty liver disease
por: Han, Mei, et al.
Publicado: (2020)

Nonalcoholic Fatty Liver Disease in Children and Adolescents Taking Atypical Antipsychotic Medications: Protocol for a Systematic Review and Meta-analysis
por: Hatem, Reem, et al.
Publicado: (2022)

Structural and functional hepatocyte polarity and liver disease
por: Gissen, Paul, et al.
Publicado: (2015)

Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease
por: Breher-Esch, Stephanie, et al.
Publicado: (2018)

Breast cancer PDxO cultures for drug discovery and functional precision oncology
por: Scherer, Sandra D., et al.
Publicado: (2023)

Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells
por: Lyall, Marcus J., et al.
Publicado: (2018)

Microbiota, type 2 diabetes and non-alcoholic fatty liver disease: protocol of an observational study
por: Motta, Benedetta M., et al.
Publicado: (2019)

Hepatoprotective effect of calculus bovis sativus on nonalcoholic fatty liver disease in mice by inhibiting oxidative stress and apoptosis of hepatocytes
por: He, Wenxi, et al.
Publicado: (2017)

Inhibition of phospholipase D1 ameliorates hepatocyte steatosis and non-alcoholic fatty liver disease
por: Wang, Huan, et al.
Publicado: (2023)

Tcf7l2 in hepatocytes regulates de novo lipogenesis in diet-induced non-alcoholic fatty liver disease in mice
por: Lee, Da Som, et al.
Publicado: (2023)

Effects of Different Cold Preservation Solutions on the Functions of Cultured Isolated Human Hepatocytes
por: Hossein Aghdaie, M., et al.
Publicado: (2020)

Characterization of the liver-macrophages isolated from a mixed primary culture of neonatal swine hepatocytes()
por: Kitani, Hiroshi, et al.
Publicado: (2014)

Resveratrol Modulates Desaturase Expression and Fatty Acid Composition of Cultured Hepatocytes
por: Kühn, Gianna, et al.
Publicado: (2018)

Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers
por: Liu, Xiao, et al.
Publicado: (2023)

Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
por: Ganan-Gomez, Irene, et al.
Publicado: (2022)

The Fatty Liver, Cirrhosis, and Liver Cancer Study (TENDENCY): Protocol for a Multicenter Case-Control Study
por: Hussain, Yaqza, et al.
Publicado: (2023)

Diet induces hepatocyte protection in fatty liver disease via modulation of PTEN signaling
por: Ikeda, Yuka, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_7ppp7t7mf3655mqhaa9255qhbo