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Differential Centrifugation to Enrich Bacterial Ribonucleoprotein Bodies (BR bodies) from Caulobacter crescentus

Bacterial RNP bodies (BR bodies) contain the mRNA decay machinery, but the collection of associated RNAs and proteins are poorly defined. Here, we present a protocol for the rapid differential centrifugation-based enrichment of BR bodies from Caulobacter crescentus cells. As native BR bodies are hig...

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Detalles Bibliográficos
Autores principales: Muthunayake, Nisansala S., Al-Husini, Nadra, Schrader, Jared M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757671/
https://www.ncbi.nlm.nih.gov/pubmed/33377099
http://dx.doi.org/10.1016/j.xpro.2020.100205