Optimization of Buffer Additives for Efficient Recovery of hGM-CSF from Inclusion Bodies Using Response Surface Methodology

Overexpression of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) by Escherichia coli leads to formation of insoluble and inactive proteins, inclusion bodies. The aim of this study was to improve recovery of biologically active hGM-CSF from inclusion bodies. The effect of types, con...

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Detalles Bibliográficos
Autores principales: Ahmadian, Mina, Jahanian-Najafabadi, Ali, Akbari, Vajihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758011/
https://www.ncbi.nlm.nih.gov/pubmed/33680031
http://dx.doi.org/10.22037/ijpr.2020.1101169

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758011/
https://www.ncbi.nlm.nih.gov/pubmed/33680031
http://dx.doi.org/10.22037/ijpr.2020.1101169

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