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Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae
Random spore analysis (RSA) is a classic method in yeast genetics that allows high-throughput purification of recombinant haploid spores following specific crosses. RSA typically involves a number of steps to induce sporulation, purge vegetative cells that fail to sporulate, and disrupt the ascus wa...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759604/ https://www.ncbi.nlm.nih.gov/pubmed/33362858 http://dx.doi.org/10.3389/fgene.2020.597482 |