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Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae

Random spore analysis (RSA) is a classic method in yeast genetics that allows high-throughput purification of recombinant haploid spores following specific crosses. RSA typically involves a number of steps to induce sporulation, purge vegetative cells that fail to sporulate, and disrupt the ascus wa...

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Detalles Bibliográficos
Autores principales: Burke, Molly K., McHugh, Kaitlin M., Kutch, Ian C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759604/
https://www.ncbi.nlm.nih.gov/pubmed/33362858
http://dx.doi.org/10.3389/fgene.2020.597482