Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization

Caspase-2 is the most specific protease of all caspases and therefore highly suitable as tag removal enzyme creating an authentic N-terminus of overexpressed tagged proteins of interest. The wild type human caspase-2 is a dimer of heterodimers generated by autocatalytic processing which is required...

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Detalles Bibliográficos
Autores principales: Cserjan-Puschmann, Monika, Lingg, Nico, Engele, Petra, Kröß, Christina, Loibl, Julian, Fischer, Andreas, Bacher, Florian, Frank, Anna-Carina, Öhlknecht, Christoph, Brocard, Cécile, Oostenbrink, Chris, Berkemeyer, Matthias, Schneider, Rainer, Striedner, Gerald, Jungbauer, Alois
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760212/
https://www.ncbi.nlm.nih.gov/pubmed/33255244
http://dx.doi.org/10.3390/biom10121592

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760212/
https://www.ncbi.nlm.nih.gov/pubmed/33255244
http://dx.doi.org/10.3390/biom10121592

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