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Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content

In both humans and animals, meat not only constitutes one of the sources of protein, but also includes various water-soluble bioactive substances such as imidazole peptides (carnosine and anserine) and taurine. Previous studies demonstrated that dietary meat species could differently affect physiolo...

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Autores principales: Nakata, Rise, Sato, Mikako, Tomonaga, Shozo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763243/
https://www.ncbi.nlm.nih.gov/pubmed/33317193
http://dx.doi.org/10.3390/metabo10120503
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author Nakata, Rise
Sato, Mikako
Tomonaga, Shozo
author_facet Nakata, Rise
Sato, Mikako
Tomonaga, Shozo
author_sort Nakata, Rise
collection PubMed
description In both humans and animals, meat not only constitutes one of the sources of protein, but also includes various water-soluble bioactive substances such as imidazole peptides (carnosine and anserine) and taurine. Previous studies demonstrated that dietary meat species could differently affect physiological functions; however, the mechanisms of this remain unclear. To explore the physiological effects of dietary defatted meat species, especially on metabolism, we investigated their influence on the metabolomic profiles of the liver, gastrocnemius muscle, and cecal content in mice. Casein (control) or a defatted meat species (beef leg, pork leg, chicken leg, or chicken breast) was supplied as the major protein source in the diet for four weeks, and metabolism-related molecules were measured by gas chromatography–mass spectrometry. We found that various metabolite levels in tissues and cecal content differed according to the types of dietary protein consumed. Specifically, differences in carnosine, 1,5-anhydro-glucitol, inositol, butyric acid, and propionic acid were clearly observed. Among them, the highest carnosine intake by dietary pork leg was clearly related to the highest carnosine level in the liver. In addition, taurine intake was suggested to be linked to some metabolic pathways including taurine and hypotaurine metabolism in cecal content. These results provide additional knowledge of the effects of different dietary meat species on human and animal health.
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spelling pubmed-77632432020-12-27 Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content Nakata, Rise Sato, Mikako Tomonaga, Shozo Metabolites Article In both humans and animals, meat not only constitutes one of the sources of protein, but also includes various water-soluble bioactive substances such as imidazole peptides (carnosine and anserine) and taurine. Previous studies demonstrated that dietary meat species could differently affect physiological functions; however, the mechanisms of this remain unclear. To explore the physiological effects of dietary defatted meat species, especially on metabolism, we investigated their influence on the metabolomic profiles of the liver, gastrocnemius muscle, and cecal content in mice. Casein (control) or a defatted meat species (beef leg, pork leg, chicken leg, or chicken breast) was supplied as the major protein source in the diet for four weeks, and metabolism-related molecules were measured by gas chromatography–mass spectrometry. We found that various metabolite levels in tissues and cecal content differed according to the types of dietary protein consumed. Specifically, differences in carnosine, 1,5-anhydro-glucitol, inositol, butyric acid, and propionic acid were clearly observed. Among them, the highest carnosine intake by dietary pork leg was clearly related to the highest carnosine level in the liver. In addition, taurine intake was suggested to be linked to some metabolic pathways including taurine and hypotaurine metabolism in cecal content. These results provide additional knowledge of the effects of different dietary meat species on human and animal health. MDPI 2020-12-09 /pmc/articles/PMC7763243/ /pubmed/33317193 http://dx.doi.org/10.3390/metabo10120503 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nakata, Rise
Sato, Mikako
Tomonaga, Shozo
Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title_full Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title_fullStr Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title_full_unstemmed Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title_short Effects of Dietary Defatted Meat Species on Metabolomic Profiles of Murine Liver, Gastrocnemius Muscle, and Cecal Content
title_sort effects of dietary defatted meat species on metabolomic profiles of murine liver, gastrocnemius muscle, and cecal content
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763243/
https://www.ncbi.nlm.nih.gov/pubmed/33317193
http://dx.doi.org/10.3390/metabo10120503
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