Autochthonous Yeast from Pork and Game Meat Fermented Sausages for Application in Meat Protection and Aroma Developing

SIMPLE SUMMARY: Yeasts are microorganisms presented naturally in meat products microbiota, which carry out an essential role in the maturation process of fermented sausages. The maturation of this type of product could be better controlled by the addition of a starter culture or a mix of strains that could standardize the process in diverse factories. Moreover, it would be favorable to find cultures that, not only conduct the curing process, but also present an added value such as the meat product protection (antioxidant capability and biocontrol activity). Yeasts also have an important role in the meat product aroma development. Therefore, it would be interesting to select a pleasant-aroma forming strain in order to elaborate a future starter culture for fermented sausage maturation. ABSTRACT: The wild yeast community was studied in fermented sausages from pork and game meat (deer and wild boar) during the maturation process from different curing rooms. Although the biotechnological importance of yeasts in the maturation process of pork sausages is known, there is a lack of information for sausage maturation involving game meat. A total of 123 yeasts were isolated and, by amplifying and sequencing of the ITS region, were classified in 14 species. Debaryomyces hansenii, Kazachstania servazzii, and Wickerhamomyces anomalus were isolated in both pork and game samples. The PCR-RAPD technique differentiated between 26 and 18 strains from pork and game meat sausages, respectively. The physicochemical parameters and their relationship with the yeast community were also studied. The antioxidant and anti-lipid peroxidation capability were analyzed and the 70% and 50% of the tested strains showed these abilities, respectively. Moreover, the biocontrol capability against mycotoxigenic molds was found in 19 strains, but better results were observed in game meat yeasts. On the other hand, almost 30% of strains produce a pleasant olfactory aroma, and volatile compounds associated with the yeast pathway metabolic during the maturation process have been characterized such as esters, aldehydes, fusel alcohols, etc. This study has allowed a better understanding of the biodiversity of this type of food, as well as selecting potential yeast strains for their future use as starters.