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In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols

Mango (Mangifera indica L.), a fruit with sensorial attractiveness and extraordinary nutritional and phytochemical composition, is one of the most consumed tropical varieties in the world. A growing body of evidence suggests that their bioactive composition differentiates them from other fruits, wit...

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Autores principales: Ordoñez-Díaz, José Luis, Moreno-Ortega, Alicia, Roldán-Guerra, Francisco Javier, Ortíz-Somovilla, Victor, Moreno-Rojas, José Manuel, Pereira-Caro, Gema
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764420/
https://www.ncbi.nlm.nih.gov/pubmed/33321767
http://dx.doi.org/10.3390/foods9121836
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author Ordoñez-Díaz, José Luis
Moreno-Ortega, Alicia
Roldán-Guerra, Francisco Javier
Ortíz-Somovilla, Victor
Moreno-Rojas, José Manuel
Pereira-Caro, Gema
author_facet Ordoñez-Díaz, José Luis
Moreno-Ortega, Alicia
Roldán-Guerra, Francisco Javier
Ortíz-Somovilla, Victor
Moreno-Rojas, José Manuel
Pereira-Caro, Gema
author_sort Ordoñez-Díaz, José Luis
collection PubMed
description Mango (Mangifera indica L.), a fruit with sensorial attractiveness and extraordinary nutritional and phytochemical composition, is one of the most consumed tropical varieties in the world. A growing body of evidence suggests that their bioactive composition differentiates them from other fruits, with mango pulp being an especially rich and diverse source of polyphenols. In this study, mango pulp polyphenols were submitted to in vitro gastrointestinal digestion and colonic fermentation, and aliquots were analyzed by HPLC-HRMS. The main phenolic compounds identified in the mango pulp were hydroxybenzoic acid-hexoside, two mono-galloyl-glucoside isomers and vanillic acid. The release of total polyphenols increased after the in vitro digestion, with an overall bioaccessibility of 206.3%. Specifically, the most bioaccessible mango polyphenols were gallic acid, 3-O-methylgallic acid, two hydroxybenzoic acid hexosides, methyl gallate, 3,4-dihydroxybenzoic acid and benzoic acid, which potentially cross the small intestine reaching the colon for fermentation by the resident microbiota. After 48 h of fecal fermentation, the main resultant mango catabolites were pyrogallol, gallic and 3,4-dihydroxybenzoic acids. This highlighted the extensive transformation of mango pulp polyphenols through the gastrointestinal tract and by the resident gut microbiota, with the resultant formation of mainly simple phenolics, which can be considered as biomarkers of the colonic metabolism of mango.
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spelling pubmed-77644202020-12-27 In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols Ordoñez-Díaz, José Luis Moreno-Ortega, Alicia Roldán-Guerra, Francisco Javier Ortíz-Somovilla, Victor Moreno-Rojas, José Manuel Pereira-Caro, Gema Foods Article Mango (Mangifera indica L.), a fruit with sensorial attractiveness and extraordinary nutritional and phytochemical composition, is one of the most consumed tropical varieties in the world. A growing body of evidence suggests that their bioactive composition differentiates them from other fruits, with mango pulp being an especially rich and diverse source of polyphenols. In this study, mango pulp polyphenols were submitted to in vitro gastrointestinal digestion and colonic fermentation, and aliquots were analyzed by HPLC-HRMS. The main phenolic compounds identified in the mango pulp were hydroxybenzoic acid-hexoside, two mono-galloyl-glucoside isomers and vanillic acid. The release of total polyphenols increased after the in vitro digestion, with an overall bioaccessibility of 206.3%. Specifically, the most bioaccessible mango polyphenols were gallic acid, 3-O-methylgallic acid, two hydroxybenzoic acid hexosides, methyl gallate, 3,4-dihydroxybenzoic acid and benzoic acid, which potentially cross the small intestine reaching the colon for fermentation by the resident microbiota. After 48 h of fecal fermentation, the main resultant mango catabolites were pyrogallol, gallic and 3,4-dihydroxybenzoic acids. This highlighted the extensive transformation of mango pulp polyphenols through the gastrointestinal tract and by the resident gut microbiota, with the resultant formation of mainly simple phenolics, which can be considered as biomarkers of the colonic metabolism of mango. MDPI 2020-12-10 /pmc/articles/PMC7764420/ /pubmed/33321767 http://dx.doi.org/10.3390/foods9121836 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ordoñez-Díaz, José Luis
Moreno-Ortega, Alicia
Roldán-Guerra, Francisco Javier
Ortíz-Somovilla, Victor
Moreno-Rojas, José Manuel
Pereira-Caro, Gema
In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title_full In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title_fullStr In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title_full_unstemmed In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title_short In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols
title_sort in vitro gastrointestinal digestion and colonic catabolism of mango (mangifera indica l.) pulp polyphenols
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764420/
https://www.ncbi.nlm.nih.gov/pubmed/33321767
http://dx.doi.org/10.3390/foods9121836
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