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Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease

Huntington’s disease (HD) is a genetic neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by massive neuronal degeneration in the striatum. In this study, we utilized solid lipid curcumin particles (SLCPs) and solid lipid particles (SLPs) to test thei...

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Autores principales: Gharaibeh, Abeer, Maiti, Panchanan, Culver, Rebecca, Heileman, Shiela, Srinageshwar, Bhairavi, Story, Darren, Spelde, Kristin, Paladugu, Leela, Munro, Nikolas, Muhn, Nathan, Kolli, Nivya, Rossignol, Julien, Dunbar, Gary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765279/
https://www.ncbi.nlm.nih.gov/pubmed/33333883
http://dx.doi.org/10.3390/ijms21249542
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author Gharaibeh, Abeer
Maiti, Panchanan
Culver, Rebecca
Heileman, Shiela
Srinageshwar, Bhairavi
Story, Darren
Spelde, Kristin
Paladugu, Leela
Munro, Nikolas
Muhn, Nathan
Kolli, Nivya
Rossignol, Julien
Dunbar, Gary L.
author_facet Gharaibeh, Abeer
Maiti, Panchanan
Culver, Rebecca
Heileman, Shiela
Srinageshwar, Bhairavi
Story, Darren
Spelde, Kristin
Paladugu, Leela
Munro, Nikolas
Muhn, Nathan
Kolli, Nivya
Rossignol, Julien
Dunbar, Gary L.
author_sort Gharaibeh, Abeer
collection PubMed
description Huntington’s disease (HD) is a genetic neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by massive neuronal degeneration in the striatum. In this study, we utilized solid lipid curcumin particles (SLCPs) and solid lipid particles (SLPs) to test their efficacy in reducing deficits in YAC128 HD mice. Eleven-month-old YAC128 male and female mice were treated orally with SLCPs (100 mg/kg) or equivalent volumes of SLPs or vehicle (phosphate-buffered saline) every other day for eight weeks. Learning and memory performance was assessed using an active-avoidance task on week eight. The mice were euthanized, and their brains were processed using Golgi-Cox staining to study the morphology of medium spiny neurons (MSNs) and Western blots to quantify amounts of DARPP-32, brain-derived neurotrophic factor (BDNF), TrkB, synaptophysin, and PSD-95. We found that both SLCPs and SLPs improved learning and memory in HD mice, as measured by the active avoidance task. We also found that SLCP and SLP treatments preserved MSNs arborization and spinal density and modulated synaptic proteins. Our study shows that SLCPs, as well as the lipid particles, can have therapeutic effects in old YAC128 HD mice in terms of recovering from HD brain pathology and cognitive deficits.
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spelling pubmed-77652792020-12-27 Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease Gharaibeh, Abeer Maiti, Panchanan Culver, Rebecca Heileman, Shiela Srinageshwar, Bhairavi Story, Darren Spelde, Kristin Paladugu, Leela Munro, Nikolas Muhn, Nathan Kolli, Nivya Rossignol, Julien Dunbar, Gary L. Int J Mol Sci Article Huntington’s disease (HD) is a genetic neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by massive neuronal degeneration in the striatum. In this study, we utilized solid lipid curcumin particles (SLCPs) and solid lipid particles (SLPs) to test their efficacy in reducing deficits in YAC128 HD mice. Eleven-month-old YAC128 male and female mice were treated orally with SLCPs (100 mg/kg) or equivalent volumes of SLPs or vehicle (phosphate-buffered saline) every other day for eight weeks. Learning and memory performance was assessed using an active-avoidance task on week eight. The mice were euthanized, and their brains were processed using Golgi-Cox staining to study the morphology of medium spiny neurons (MSNs) and Western blots to quantify amounts of DARPP-32, brain-derived neurotrophic factor (BDNF), TrkB, synaptophysin, and PSD-95. We found that both SLCPs and SLPs improved learning and memory in HD mice, as measured by the active avoidance task. We also found that SLCP and SLP treatments preserved MSNs arborization and spinal density and modulated synaptic proteins. Our study shows that SLCPs, as well as the lipid particles, can have therapeutic effects in old YAC128 HD mice in terms of recovering from HD brain pathology and cognitive deficits. MDPI 2020-12-15 /pmc/articles/PMC7765279/ /pubmed/33333883 http://dx.doi.org/10.3390/ijms21249542 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gharaibeh, Abeer
Maiti, Panchanan
Culver, Rebecca
Heileman, Shiela
Srinageshwar, Bhairavi
Story, Darren
Spelde, Kristin
Paladugu, Leela
Munro, Nikolas
Muhn, Nathan
Kolli, Nivya
Rossignol, Julien
Dunbar, Gary L.
Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title_full Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title_fullStr Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title_full_unstemmed Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title_short Solid Lipid Curcumin Particles Protect Medium Spiny Neuronal Morphology, and Reduce Learning and Memory Deficits in the YAC128 Mouse Model of Huntington’s Disease
title_sort solid lipid curcumin particles protect medium spiny neuronal morphology, and reduce learning and memory deficits in the yac128 mouse model of huntington’s disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765279/
https://www.ncbi.nlm.nih.gov/pubmed/33333883
http://dx.doi.org/10.3390/ijms21249542
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