Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells

Baculovirus expression vectors are successfully used for the commercial production of complex (glyco)proteins in eukaryotic cells. The genome engineering of single-copy baculovirus infectious clones (bacmids) in E. coli has been valuable in the study of baculovirus biology, but bacmids are not yet w...

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Detalles Bibliográficos
Autores principales: Pijlman, Gorben P., Grose, Carissa, Hick, Tessy A. H., Breukink, Herman E., van den Braak, Robin, Abbo, Sandra R., Geertsema, Corinne, van Oers, Monique M., Martens, Dirk E., Esposito, Dominic
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765880/
https://www.ncbi.nlm.nih.gov/pubmed/33339324
http://dx.doi.org/10.3390/v12121448

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765880/
https://www.ncbi.nlm.nih.gov/pubmed/33339324
http://dx.doi.org/10.3390/v12121448

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