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Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit
Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766571/ https://www.ncbi.nlm.nih.gov/pubmed/33348687 http://dx.doi.org/10.3390/antiox9121295 |
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author | Afonso, Sílvia Oliveira, Ivo Vaz Meyer, Anne S. Aires, Alfredo Saavedra, Maria José Gonçalves, Berta |
author_facet | Afonso, Sílvia Oliveira, Ivo Vaz Meyer, Anne S. Aires, Alfredo Saavedra, Maria José Gonçalves, Berta |
author_sort | Afonso, Sílvia |
collection | PubMed |
description | Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties. |
format | Online Article Text |
id | pubmed-7766571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77665712020-12-28 Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit Afonso, Sílvia Oliveira, Ivo Vaz Meyer, Anne S. Aires, Alfredo Saavedra, Maria José Gonçalves, Berta Antioxidants (Basel) Article Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties. MDPI 2020-12-17 /pmc/articles/PMC7766571/ /pubmed/33348687 http://dx.doi.org/10.3390/antiox9121295 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Afonso, Sílvia Oliveira, Ivo Vaz Meyer, Anne S. Aires, Alfredo Saavedra, Maria José Gonçalves, Berta Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title | Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title_full | Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title_fullStr | Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title_full_unstemmed | Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title_short | Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit |
title_sort | phenolic profile and bioactive potential of stems and seed kernels of sweet cherry fruit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766571/ https://www.ncbi.nlm.nih.gov/pubmed/33348687 http://dx.doi.org/10.3390/antiox9121295 |
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