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Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection

This study aims to discriminate fresh fish from frozen/thawed by identification of the key metabolites that are altered during the freezing/thawing processing. Atlantic salmon (Salmo salar) and bullet tuna (Auxis rochei) were selected as they are representative of broad consumption, and susceptible...

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Autores principales: Chiesa, Luca Maria, Pavlovic, Radmila, Nobile, Maria, Di Cesare, Federica, Malandra, Renato, Pessina, Davide, Panseri, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766691/
https://www.ncbi.nlm.nih.gov/pubmed/33353233
http://dx.doi.org/10.3390/foods9121896
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author Chiesa, Luca Maria
Pavlovic, Radmila
Nobile, Maria
Di Cesare, Federica
Malandra, Renato
Pessina, Davide
Panseri, Sara
author_facet Chiesa, Luca Maria
Pavlovic, Radmila
Nobile, Maria
Di Cesare, Federica
Malandra, Renato
Pessina, Davide
Panseri, Sara
author_sort Chiesa, Luca Maria
collection PubMed
description This study aims to discriminate fresh fish from frozen/thawed by identification of the key metabolites that are altered during the freezing/thawing processing. Atlantic salmon (Salmo salar) and bullet tuna (Auxis rochei) were selected as they are representative of broad consumption, and susceptible to pathogen contamination. Atlantic salmon samples were subjected to the following regimes: −20 °C (24h) and −35 °C (15 h) freezing, then thawed respectively in the blast chiller and in the cold room and analyzed immediately or after 10 days; (2) bullet tuna samples were frozen at −18 °C and thawed after 15, 30 and 90 days. High resolution mass spectrometry based on untargeted metabolomic analyses and statistical data treatment confirmed significant variations in the quantity of certain metabolites: the amount of l-phenylalanine in salmon increased immediately after thawing while that of anserine decreased. The concentration of l-arginine and its metabolites was altered at the 10th day after thawing rendering them promising markers of salmon freezing/thawing. As regards bullet tuna, compounds resulting from lipid degradation (l-α-Glyceryl-phosphoryl-choline and N-methyl-ethanolamine phosphate) increased notably during the storage period. This approach could be used to reveal common fraudulent incidents such as deliberate replacement of fresh fish with frozen/thawed, with food safety risks as the primary implication.
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spelling pubmed-77666912020-12-28 Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection Chiesa, Luca Maria Pavlovic, Radmila Nobile, Maria Di Cesare, Federica Malandra, Renato Pessina, Davide Panseri, Sara Foods Article This study aims to discriminate fresh fish from frozen/thawed by identification of the key metabolites that are altered during the freezing/thawing processing. Atlantic salmon (Salmo salar) and bullet tuna (Auxis rochei) were selected as they are representative of broad consumption, and susceptible to pathogen contamination. Atlantic salmon samples were subjected to the following regimes: −20 °C (24h) and −35 °C (15 h) freezing, then thawed respectively in the blast chiller and in the cold room and analyzed immediately or after 10 days; (2) bullet tuna samples were frozen at −18 °C and thawed after 15, 30 and 90 days. High resolution mass spectrometry based on untargeted metabolomic analyses and statistical data treatment confirmed significant variations in the quantity of certain metabolites: the amount of l-phenylalanine in salmon increased immediately after thawing while that of anserine decreased. The concentration of l-arginine and its metabolites was altered at the 10th day after thawing rendering them promising markers of salmon freezing/thawing. As regards bullet tuna, compounds resulting from lipid degradation (l-α-Glyceryl-phosphoryl-choline and N-methyl-ethanolamine phosphate) increased notably during the storage period. This approach could be used to reveal common fraudulent incidents such as deliberate replacement of fresh fish with frozen/thawed, with food safety risks as the primary implication. MDPI 2020-12-18 /pmc/articles/PMC7766691/ /pubmed/33353233 http://dx.doi.org/10.3390/foods9121896 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiesa, Luca Maria
Pavlovic, Radmila
Nobile, Maria
Di Cesare, Federica
Malandra, Renato
Pessina, Davide
Panseri, Sara
Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title_full Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title_fullStr Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title_full_unstemmed Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title_short Discrimination between Fresh and Frozen-Thawed Fish Involved in Food Safety and Fraud Protection
title_sort discrimination between fresh and frozen-thawed fish involved in food safety and fraud protection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766691/
https://www.ncbi.nlm.nih.gov/pubmed/33353233
http://dx.doi.org/10.3390/foods9121896
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