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Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycl...

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Detalles Bibliográficos
Autores principales: KOBAYASHI, Mitsuru, ITO, Jun, SHIRASUNA, Koumei, KUWAYAMA, Takehito, IWATA, Hisataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768170/
https://www.ncbi.nlm.nih.gov/pubmed/32908082
http://dx.doi.org/10.1262/jrd.2020-097