Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus

SARS‐CoV‐2 entry is mediated by binding of the spike protein (S) to the surface receptor ACE2 and subsequent priming by host TMPRSS2 allowing membrane fusion. Here, we produced extracellular vesicles (EVs) exposing ACE2 and demonstrate that ACE2‐EVs are efficient decoys for SARS‐CoV‐2 S protein‐cont...

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Autores principales: Cocozza, Federico, Névo, Nathalie, Piovesana, Ester, Lahaye, Xavier, Buchrieser, Julian, Schwartz, Olivier, Manel, Nicolas, Tkach, Mercedes, Théry, Clotilde, Martin‐Jaular, Lorena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769856/
https://www.ncbi.nlm.nih.gov/pubmed/33391636
http://dx.doi.org/10.1002/jev2.12050
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author Cocozza, Federico
Névo, Nathalie
Piovesana, Ester
Lahaye, Xavier
Buchrieser, Julian
Schwartz, Olivier
Manel, Nicolas
Tkach, Mercedes
Théry, Clotilde
Martin‐Jaular, Lorena
author_facet Cocozza, Federico
Névo, Nathalie
Piovesana, Ester
Lahaye, Xavier
Buchrieser, Julian
Schwartz, Olivier
Manel, Nicolas
Tkach, Mercedes
Théry, Clotilde
Martin‐Jaular, Lorena
author_sort Cocozza, Federico
collection PubMed
description SARS‐CoV‐2 entry is mediated by binding of the spike protein (S) to the surface receptor ACE2 and subsequent priming by host TMPRSS2 allowing membrane fusion. Here, we produced extracellular vesicles (EVs) exposing ACE2 and demonstrate that ACE2‐EVs are efficient decoys for SARS‐CoV‐2 S protein‐containing lentivirus. Reduction of infectivity positively correlates with the level of ACE2, is much more efficient than with soluble ACE2 and further enhanced by the inclusion of TMPRSS2.
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spelling pubmed-77698562020-12-31 Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus Cocozza, Federico Névo, Nathalie Piovesana, Ester Lahaye, Xavier Buchrieser, Julian Schwartz, Olivier Manel, Nicolas Tkach, Mercedes Théry, Clotilde Martin‐Jaular, Lorena J Extracell Vesicles Short Communication SARS‐CoV‐2 entry is mediated by binding of the spike protein (S) to the surface receptor ACE2 and subsequent priming by host TMPRSS2 allowing membrane fusion. Here, we produced extracellular vesicles (EVs) exposing ACE2 and demonstrate that ACE2‐EVs are efficient decoys for SARS‐CoV‐2 S protein‐containing lentivirus. Reduction of infectivity positively correlates with the level of ACE2, is much more efficient than with soluble ACE2 and further enhanced by the inclusion of TMPRSS2. John Wiley and Sons Inc. 2020-12-28 2020-12 /pmc/articles/PMC7769856/ /pubmed/33391636 http://dx.doi.org/10.1002/jev2.12050 Text en © 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Cocozza, Federico
Névo, Nathalie
Piovesana, Ester
Lahaye, Xavier
Buchrieser, Julian
Schwartz, Olivier
Manel, Nicolas
Tkach, Mercedes
Théry, Clotilde
Martin‐Jaular, Lorena
Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title_full Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title_fullStr Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title_full_unstemmed Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title_short Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
title_sort extracellular vesicles containing ace2 efficiently prevent infection by sars‐cov‐2 spike protein‐containing virus
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769856/
https://www.ncbi.nlm.nih.gov/pubmed/33391636
http://dx.doi.org/10.1002/jev2.12050
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