Combined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cells

The CRISPR/Cas9 system is a versatile tool for functional genomics and forward genetic screens in mammalian cells. However, it has been challenging to deliver the CRISPR components to sensitive cell types, such as primary human hematopoietic stem and progenitor cells (HSPCs), partly due to lentivira...

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Detalles Bibliográficos
Autores principales: Yudovich, David, Bäckström, Alexandra, Schmiderer, Ludwig, Žemaitis, Kristijonas, Subramaniam, Agatheeswaran, Larsson, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769964/
https://www.ncbi.nlm.nih.gov/pubmed/33372184
http://dx.doi.org/10.1038/s41598-020-79724-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769964/
https://www.ncbi.nlm.nih.gov/pubmed/33372184
http://dx.doi.org/10.1038/s41598-020-79724-x

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