Cargando…

Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage

In articular cartilage-repair, grafts usually fuse unsatisfactorily with surrounding host cartilage. Enzymatic dissociation of cartilaginous matrix to free chondrocytes may benefit fusion. We tested such a hypothesis with human cartilage in vitro, and with porcine cartilage in vivo. Human articular...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Ching-Chuan, Hsieh, Chang-Hsun, Liao, Chun-Jen, Chang, Wen-Hsiang, Liao, Wei-Ju, Tsai-Wu, Jyy-Jih, Chiang, Hongsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770344/
https://www.ncbi.nlm.nih.gov/pubmed/33426207
http://dx.doi.org/10.1016/j.reth.2020.05.006
_version_ 1783629487698608128
author Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Liao, Chun-Jen
Chang, Wen-Hsiang
Liao, Wei-Ju
Tsai-Wu, Jyy-Jih
Chiang, Hongsen
author_facet Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Liao, Chun-Jen
Chang, Wen-Hsiang
Liao, Wei-Ju
Tsai-Wu, Jyy-Jih
Chiang, Hongsen
author_sort Jiang, Ching-Chuan
collection PubMed
description In articular cartilage-repair, grafts usually fuse unsatisfactorily with surrounding host cartilage. Enzymatic dissociation of cartilaginous matrix to free chondrocytes may benefit fusion. We tested such a hypothesis with human cartilage in vitro, and with porcine cartilage in vivo. Human articular cartilage was collected from knee surgeries, cut into disc-and-ring sets, and randomly distributed into three groups: disc-and-ring sets in Group 1 were left untreated; in Group 2 only discs, and in Group 3 both discs and rings were treated with enzyme. Each disc-and-ring reassembly was cultured in a perfusion system for 14 days; expression of cartilage marker proteins and genes was evaluated by immunohistochemistry and PCR. Porcine articular cartilage from knees was similarly fashioned into disc-and-ring combinations. Specimens were randomly distributed into a control group without further treatment, and an experimental group with both disc and ring treated with enzyme. Each disc-and-ring reassembly was transplanted into subcutaneous space of a nude mouse for 30 days, and retrieved to examine disc-ring interface. In in vitro study with human cartilage, a visible gap remained at disc-ring interfaces in Group 1, yet became indiscernible in Group 2 and 3. Marker genes, including type II collagen, aggrecan and Sox 9, were well expressed by chondrocytes in all specimens, indicating that chondrocytes’ phenotype retained regardless of enzymatic treatment. Similar results were found inin vivo study with porcine cartilage. Enzymatic dissociation of cartilaginous matrix promotes fusion of adjacent cartilage. The clinical relevance may be a novel method to facilitate integration of repaired cartilage in joints.
format Online
Article
Text
id pubmed-7770344
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Japanese Society for Regenerative Medicine
record_format MEDLINE/PubMed
spelling pubmed-77703442021-01-08 Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage Jiang, Ching-Chuan Hsieh, Chang-Hsun Liao, Chun-Jen Chang, Wen-Hsiang Liao, Wei-Ju Tsai-Wu, Jyy-Jih Chiang, Hongsen Regen Ther Original Article In articular cartilage-repair, grafts usually fuse unsatisfactorily with surrounding host cartilage. Enzymatic dissociation of cartilaginous matrix to free chondrocytes may benefit fusion. We tested such a hypothesis with human cartilage in vitro, and with porcine cartilage in vivo. Human articular cartilage was collected from knee surgeries, cut into disc-and-ring sets, and randomly distributed into three groups: disc-and-ring sets in Group 1 were left untreated; in Group 2 only discs, and in Group 3 both discs and rings were treated with enzyme. Each disc-and-ring reassembly was cultured in a perfusion system for 14 days; expression of cartilage marker proteins and genes was evaluated by immunohistochemistry and PCR. Porcine articular cartilage from knees was similarly fashioned into disc-and-ring combinations. Specimens were randomly distributed into a control group without further treatment, and an experimental group with both disc and ring treated with enzyme. Each disc-and-ring reassembly was transplanted into subcutaneous space of a nude mouse for 30 days, and retrieved to examine disc-ring interface. In in vitro study with human cartilage, a visible gap remained at disc-ring interfaces in Group 1, yet became indiscernible in Group 2 and 3. Marker genes, including type II collagen, aggrecan and Sox 9, were well expressed by chondrocytes in all specimens, indicating that chondrocytes’ phenotype retained regardless of enzymatic treatment. Similar results were found inin vivo study with porcine cartilage. Enzymatic dissociation of cartilaginous matrix promotes fusion of adjacent cartilage. The clinical relevance may be a novel method to facilitate integration of repaired cartilage in joints. Japanese Society for Regenerative Medicine 2020-07-28 /pmc/articles/PMC7770344/ /pubmed/33426207 http://dx.doi.org/10.1016/j.reth.2020.05.006 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Liao, Chun-Jen
Chang, Wen-Hsiang
Liao, Wei-Ju
Tsai-Wu, Jyy-Jih
Chiang, Hongsen
Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title_full Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title_fullStr Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title_full_unstemmed Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title_short Collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
title_sort collagenase treatment of cartilaginous matrix promotes fusion of adjacent cartilage
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770344/
https://www.ncbi.nlm.nih.gov/pubmed/33426207
http://dx.doi.org/10.1016/j.reth.2020.05.006
work_keys_str_mv AT jiangchingchuan collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT hsiehchanghsun collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT liaochunjen collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT changwenhsiang collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT liaoweiju collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT tsaiwujyyjih collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage
AT chianghongsen collagenasetreatmentofcartilaginousmatrixpromotesfusionofadjacentcartilage