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MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression

The severity of sepsis may be associated with excessive inflammation, thus leading to acute liver injury. MicroRNA-21 is highly expressed in the liver of a variety of inflammation-related diseases, and PPARα is also proved to participate in regulating inflammation. In the present study, the LPS-indu...

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Autores principales: Du, Xianjin, Wu, Miao, Tian, Dan, Zhou, Jianlin, Wang, Lu, Zhan, Liying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772039/
https://www.ncbi.nlm.nih.gov/pubmed/33424957
http://dx.doi.org/10.1155/2020/6633022
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author Du, Xianjin
Wu, Miao
Tian, Dan
Zhou, Jianlin
Wang, Lu
Zhan, Liying
author_facet Du, Xianjin
Wu, Miao
Tian, Dan
Zhou, Jianlin
Wang, Lu
Zhan, Liying
author_sort Du, Xianjin
collection PubMed
description The severity of sepsis may be associated with excessive inflammation, thus leading to acute liver injury. MicroRNA-21 is highly expressed in the liver of a variety of inflammation-related diseases, and PPARα is also proved to participate in regulating inflammation. In the present study, the LPS-induced sepsis model was established. We found that microRNA-21 expression was upregulated in the liver of sepsis mice, and microRNA-21 inhibition significantly reduced the liver injury. The expression of liver injury markers, inflammation cytokines, and PPARα in the septic mice was higher than in antagomir-21 treated septic mice. In addition, we also found that PPARα is the target gene of microRNA-21; PPARα antagonist GW6471 could reverse the effect of antagomir-21. In conclusion, our study illustrated that microRNA-21 exacerbate acute liver injury in sepsis mice by inhibiting PPARα expression.
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spelling pubmed-77720392021-01-08 MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression Du, Xianjin Wu, Miao Tian, Dan Zhou, Jianlin Wang, Lu Zhan, Liying PPAR Res Research Article The severity of sepsis may be associated with excessive inflammation, thus leading to acute liver injury. MicroRNA-21 is highly expressed in the liver of a variety of inflammation-related diseases, and PPARα is also proved to participate in regulating inflammation. In the present study, the LPS-induced sepsis model was established. We found that microRNA-21 expression was upregulated in the liver of sepsis mice, and microRNA-21 inhibition significantly reduced the liver injury. The expression of liver injury markers, inflammation cytokines, and PPARα in the septic mice was higher than in antagomir-21 treated septic mice. In addition, we also found that PPARα is the target gene of microRNA-21; PPARα antagonist GW6471 could reverse the effect of antagomir-21. In conclusion, our study illustrated that microRNA-21 exacerbate acute liver injury in sepsis mice by inhibiting PPARα expression. Hindawi 2020-12-22 /pmc/articles/PMC7772039/ /pubmed/33424957 http://dx.doi.org/10.1155/2020/6633022 Text en Copyright © 2020 Xianjin Du et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Du, Xianjin
Wu, Miao
Tian, Dan
Zhou, Jianlin
Wang, Lu
Zhan, Liying
MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title_full MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title_fullStr MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title_full_unstemmed MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title_short MicroRNA-21 Contributes to Acute Liver Injury in LPS-Induced Sepsis Mice by Inhibiting PPARα Expression
title_sort microrna-21 contributes to acute liver injury in lps-induced sepsis mice by inhibiting pparα expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772039/
https://www.ncbi.nlm.nih.gov/pubmed/33424957
http://dx.doi.org/10.1155/2020/6633022
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