Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes

The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anch...

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Autores principales: Chen, Chia-Yu, Kim, David. M., Lee, Cliff, Da Silva, John, Nagai, Shigemi, Nojiri, Toshiki, Nagai, Masazumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773734/
https://www.ncbi.nlm.nih.gov/pubmed/33380730
http://dx.doi.org/10.1038/s41368-020-00103-3
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author Chen, Chia-Yu
Kim, David. M.
Lee, Cliff
Da Silva, John
Nagai, Shigemi
Nojiri, Toshiki
Nagai, Masazumi
author_facet Chen, Chia-Yu
Kim, David. M.
Lee, Cliff
Da Silva, John
Nagai, Shigemi
Nojiri, Toshiki
Nagai, Masazumi
author_sort Chen, Chia-Yu
collection PubMed
description The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO(2) nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO(2) surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO(2) nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal.
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spelling pubmed-77737342021-01-07 Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes Chen, Chia-Yu Kim, David. M. Lee, Cliff Da Silva, John Nagai, Shigemi Nojiri, Toshiki Nagai, Masazumi Int J Oral Sci Article The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO(2) nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO(2) surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO(2) nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal. Nature Publishing Group UK 2020-12-30 /pmc/articles/PMC7773734/ /pubmed/33380730 http://dx.doi.org/10.1038/s41368-020-00103-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Chia-Yu
Kim, David. M.
Lee, Cliff
Da Silva, John
Nagai, Shigemi
Nojiri, Toshiki
Nagai, Masazumi
Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title_full Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title_fullStr Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title_full_unstemmed Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title_short Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
title_sort biological efficacy of perpendicular type-i collagen protruded from tio(2)-nanotubes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773734/
https://www.ncbi.nlm.nih.gov/pubmed/33380730
http://dx.doi.org/10.1038/s41368-020-00103-3
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