Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes
The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anch...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773734/ https://www.ncbi.nlm.nih.gov/pubmed/33380730 http://dx.doi.org/10.1038/s41368-020-00103-3 |
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author | Chen, Chia-Yu Kim, David. M. Lee, Cliff Da Silva, John Nagai, Shigemi Nojiri, Toshiki Nagai, Masazumi |
author_facet | Chen, Chia-Yu Kim, David. M. Lee, Cliff Da Silva, John Nagai, Shigemi Nojiri, Toshiki Nagai, Masazumi |
author_sort | Chen, Chia-Yu |
collection | PubMed |
description | The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO(2) nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO(2) surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO(2) nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal. |
format | Online Article Text |
id | pubmed-7773734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77737342021-01-07 Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes Chen, Chia-Yu Kim, David. M. Lee, Cliff Da Silva, John Nagai, Shigemi Nojiri, Toshiki Nagai, Masazumi Int J Oral Sci Article The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO(2) nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO(2) nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO(2) surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO(2) nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal. Nature Publishing Group UK 2020-12-30 /pmc/articles/PMC7773734/ /pubmed/33380730 http://dx.doi.org/10.1038/s41368-020-00103-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chen, Chia-Yu Kim, David. M. Lee, Cliff Da Silva, John Nagai, Shigemi Nojiri, Toshiki Nagai, Masazumi Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title | Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title_full | Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title_fullStr | Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title_full_unstemmed | Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title_short | Biological efficacy of perpendicular type-I collagen protruded from TiO(2)-nanotubes |
title_sort | biological efficacy of perpendicular type-i collagen protruded from tio(2)-nanotubes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773734/ https://www.ncbi.nlm.nih.gov/pubmed/33380730 http://dx.doi.org/10.1038/s41368-020-00103-3 |
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