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Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles

This study aimed to evaluate the effects of including microalgae Chlorella sp. or Nannochloropsis sp. in plant-based diets on antioxidant mechanisms of European sea bass (Dicentrarchus labrax) juveniles. For this purpose, three isoproteic (50%) and isolipidic (19%) diets were formulated: a practical...

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Autores principales: Castro, Carolina, Coutinho, Filipe, Iglesias, Paula, Oliva-Teles, Aires, Couto, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773920/
https://www.ncbi.nlm.nih.gov/pubmed/33392297
http://dx.doi.org/10.3389/fvets.2020.607575
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author Castro, Carolina
Coutinho, Filipe
Iglesias, Paula
Oliva-Teles, Aires
Couto, Ana
author_facet Castro, Carolina
Coutinho, Filipe
Iglesias, Paula
Oliva-Teles, Aires
Couto, Ana
author_sort Castro, Carolina
collection PubMed
description This study aimed to evaluate the effects of including microalgae Chlorella sp. or Nannochloropsis sp. in plant-based diets on antioxidant mechanisms of European sea bass (Dicentrarchus labrax) juveniles. For this purpose, three isoproteic (50%) and isolipidic (19%) diets were formulated: a practical diet, containing 15% fish meal (FM) and plant ingredients as the protein source and a mixture of fish oil and vegetable oils (40: 60) as lipid source (control diet); and two diets identical to the control but with the FM replaced by Nannochloropsis sp. or Chlorella sp. (diets Nanno and Chlo, respectively). The diets were offered to quadruplicate groups of 25 fish (initial body weight: 24 ± 1 g) for 11 weeks and then enzymatic and non-enzymatic antioxidant mechanisms and lipid oxidative biomarkers were assessed in the liver and intestine of these fish. Results showed that the antioxidant response was tissue-dependent, with the liver exhibiting lower glutathione peroxidase and glucose-6-phosphate dehydrogenase (only in Chlo group) activities, and intestine lower superoxide dismutase activity with the diets including microalgae compared to control diet. An increase of oxidized glutathione content was also observed in the intestine of fish fed the microalgae diets. Catalase and glutathione reductase activities, oxidative stress index, and total and reduced glutathione, were unaffected by dietary treatments in both tissues. Overall, the lipid peroxidation status was not compromised by the replacement of FM by microalgae.
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spelling pubmed-77739202021-01-01 Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles Castro, Carolina Coutinho, Filipe Iglesias, Paula Oliva-Teles, Aires Couto, Ana Front Vet Sci Veterinary Science This study aimed to evaluate the effects of including microalgae Chlorella sp. or Nannochloropsis sp. in plant-based diets on antioxidant mechanisms of European sea bass (Dicentrarchus labrax) juveniles. For this purpose, three isoproteic (50%) and isolipidic (19%) diets were formulated: a practical diet, containing 15% fish meal (FM) and plant ingredients as the protein source and a mixture of fish oil and vegetable oils (40: 60) as lipid source (control diet); and two diets identical to the control but with the FM replaced by Nannochloropsis sp. or Chlorella sp. (diets Nanno and Chlo, respectively). The diets were offered to quadruplicate groups of 25 fish (initial body weight: 24 ± 1 g) for 11 weeks and then enzymatic and non-enzymatic antioxidant mechanisms and lipid oxidative biomarkers were assessed in the liver and intestine of these fish. Results showed that the antioxidant response was tissue-dependent, with the liver exhibiting lower glutathione peroxidase and glucose-6-phosphate dehydrogenase (only in Chlo group) activities, and intestine lower superoxide dismutase activity with the diets including microalgae compared to control diet. An increase of oxidized glutathione content was also observed in the intestine of fish fed the microalgae diets. Catalase and glutathione reductase activities, oxidative stress index, and total and reduced glutathione, were unaffected by dietary treatments in both tissues. Overall, the lipid peroxidation status was not compromised by the replacement of FM by microalgae. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC7773920/ /pubmed/33392297 http://dx.doi.org/10.3389/fvets.2020.607575 Text en Copyright © 2020 Castro, Coutinho, Iglesias, Oliva-Teles and Couto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Castro, Carolina
Coutinho, Filipe
Iglesias, Paula
Oliva-Teles, Aires
Couto, Ana
Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title_full Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title_fullStr Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title_full_unstemmed Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title_short Chlorella sp. and Nannochloropsis sp. Inclusion in Plant-Based Diets Modulate the Intestine and Liver Antioxidant Mechanisms of European Sea Bass Juveniles
title_sort chlorella sp. and nannochloropsis sp. inclusion in plant-based diets modulate the intestine and liver antioxidant mechanisms of european sea bass juveniles
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773920/
https://www.ncbi.nlm.nih.gov/pubmed/33392297
http://dx.doi.org/10.3389/fvets.2020.607575
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