A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies

Restriction endonucleases play a central role in the microbial immune system against viruses and are widely used in DNA specific cleavage, which is called restriction digestion, for genetic engineering. Herein, we applied digital cell-free protein synthesis as an easy-to-use orthogonal readout means...

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Detalles Bibliográficos
Autores principales: Zhang, Yi, Nunoura, Takuro, Nishiura, Daisuke, Hirai, Miho, Shimamura, Shigeru, Kurosawa, Kanako, Ishiwata, Chieko, Deguchi, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775078/
https://www.ncbi.nlm.nih.gov/pubmed/33382779
http://dx.doi.org/10.1371/journal.pone.0244464

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775078/
https://www.ncbi.nlm.nih.gov/pubmed/33382779
http://dx.doi.org/10.1371/journal.pone.0244464

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