Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica

The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understanding of the pathogenesis of F. gigantica infection. I...

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Autores principales: Shi, Wei, He, Jun-Jun, Mei, Xue-Fang, Lu, Ke-Jing, Zeng, Zi-Xuan, Zhang, Yao-Yao, Sheng, Zhao-An, Elsheikha, Hany M., Huang, Wei-Yi, Zhu, Xing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775122/
https://www.ncbi.nlm.nih.gov/pubmed/33332355
http://dx.doi.org/10.1371/journal.pntd.0008951
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author Shi, Wei
He, Jun-Jun
Mei, Xue-Fang
Lu, Ke-Jing
Zeng, Zi-Xuan
Zhang, Yao-Yao
Sheng, Zhao-An
Elsheikha, Hany M.
Huang, Wei-Yi
Zhu, Xing-Quan
author_facet Shi, Wei
He, Jun-Jun
Mei, Xue-Fang
Lu, Ke-Jing
Zeng, Zi-Xuan
Zhang, Yao-Yao
Sheng, Zhao-An
Elsheikha, Hany M.
Huang, Wei-Yi
Zhu, Xing-Quan
author_sort Shi, Wei
collection PubMed
description The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understanding of the pathogenesis of F. gigantica infection. In this study, we investigated the alterations in the hepatic microRNAs (miRNAs) in mice treated with FgESPs using high-throughput small RNA (sRNA) sequencing and bioinformatics analysis. The expression of seven miRNAs was confirmed by quantitative stem-loop reverse transcription quantitative PCR (qRT-PCR). A total of 1,313 miRNAs were identified in the liver of mice, and the differentially expressed (DE) miRNAs varied across the time lapsed post exposure to FgESPs. We identified 67, 154 and 53 dysregulated miRNAs at 1, 4 and 12 weeks post-exposure, respectively. 5 miRNAs (miR-126a-3p, miR-150-5p, miR-155-5p, miR-181a-5p and miR-362-3p) were commonly dysregulated at the three time points. We also found that most of the DE miRNAs were induced by FgESPs in the mouse liver after 4 weeks of exposure. These were subjected to Gene Ontology (GO) enrichment analysis, which showed that the predicted targets of the hepatic DE miRNAs of mice 4 weeks of FgESPs injection were enriched in GO terms, including cell membrane, ion binding, cellular communication, organelle and DNA damage. KEGG analysis indicated that the predicted targets of the most downregulated miRNAs were involved in 15 neural activity-related pathways, 6 digestion-related pathways, 20 immune response-related pathways and 17 cancer-related pathways. These data provide new insights into how FgESPs can dysregulate hepatic miRNAs, which play important roles in modulating several aspects of F. gigantica pathogenesis.
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spelling pubmed-77751222021-01-07 Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica Shi, Wei He, Jun-Jun Mei, Xue-Fang Lu, Ke-Jing Zeng, Zi-Xuan Zhang, Yao-Yao Sheng, Zhao-An Elsheikha, Hany M. Huang, Wei-Yi Zhu, Xing-Quan PLoS Negl Trop Dis Research Article The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understanding of the pathogenesis of F. gigantica infection. In this study, we investigated the alterations in the hepatic microRNAs (miRNAs) in mice treated with FgESPs using high-throughput small RNA (sRNA) sequencing and bioinformatics analysis. The expression of seven miRNAs was confirmed by quantitative stem-loop reverse transcription quantitative PCR (qRT-PCR). A total of 1,313 miRNAs were identified in the liver of mice, and the differentially expressed (DE) miRNAs varied across the time lapsed post exposure to FgESPs. We identified 67, 154 and 53 dysregulated miRNAs at 1, 4 and 12 weeks post-exposure, respectively. 5 miRNAs (miR-126a-3p, miR-150-5p, miR-155-5p, miR-181a-5p and miR-362-3p) were commonly dysregulated at the three time points. We also found that most of the DE miRNAs were induced by FgESPs in the mouse liver after 4 weeks of exposure. These were subjected to Gene Ontology (GO) enrichment analysis, which showed that the predicted targets of the hepatic DE miRNAs of mice 4 weeks of FgESPs injection were enriched in GO terms, including cell membrane, ion binding, cellular communication, organelle and DNA damage. KEGG analysis indicated that the predicted targets of the most downregulated miRNAs were involved in 15 neural activity-related pathways, 6 digestion-related pathways, 20 immune response-related pathways and 17 cancer-related pathways. These data provide new insights into how FgESPs can dysregulate hepatic miRNAs, which play important roles in modulating several aspects of F. gigantica pathogenesis. Public Library of Science 2020-12-17 /pmc/articles/PMC7775122/ /pubmed/33332355 http://dx.doi.org/10.1371/journal.pntd.0008951 Text en © 2020 Shi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shi, Wei
He, Jun-Jun
Mei, Xue-Fang
Lu, Ke-Jing
Zeng, Zi-Xuan
Zhang, Yao-Yao
Sheng, Zhao-An
Elsheikha, Hany M.
Huang, Wei-Yi
Zhu, Xing-Quan
Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title_full Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title_fullStr Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title_full_unstemmed Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title_short Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica
title_sort dysregulation of hepatic microrna expression in c57bl/6 mice affected by excretory-secretory products of fasciola gigantica
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775122/
https://www.ncbi.nlm.nih.gov/pubmed/33332355
http://dx.doi.org/10.1371/journal.pntd.0008951
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